Kenya Kuchi
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Kenya Kuchi
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region Kenya
Purpose Fighting
Description

Kuchi is Asil-like ecotype indigenous to coast region of East Africa. It was likely imported from South Asia, Southeast Asia, or West Asia. It is raised for cockfighting. Now it is also involved breeding programs to improve indigenous poultry production.

Characteristics

This is a big breed and they weigh between 6-9Kg and 4-6Kg for the roosters and hens. They only lay between 8-10 eggs in their hatching season compared to 18-22 by indigenous breeds and rarely brood their eggs. Kuchi are tall and long necked with an upright stance, cushion-like comb and nonexistent wattles. It has a large rounded head with a medium sized parrot-like beak with a full and lobster-like tail whereas young ones have yellowish eyes that become pearly pale (milkish) with age.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 151027 650455 23.2187% 12664 104154 12.1589%
exonic;splicing 28 188 14.8936% 0 0 0%
exonic_unknown 2 582 0.3436% 0 82 0%
frameshift_deletion 0 0 0% 730 15977 4.5691%
frameshift_insertion 0 0 0% 620 13308 4.6589%
intergenic 3339880 15129055 22.0759% 253611 2238383 11.3301%
intronic 4227106 17735594 23.834% 343030 2641780 12.9848%
ncRNA_exonic 90641 400185 22.6498% 6364 54342 11.711%
ncRNA_exonic;splicing 58 231 25.1082% 5 43 11.6279%
ncRNA_intronic 848238 3728327 22.7512% 68892 575920 11.9621%
ncRNA_splicing 521 2341 22.2554% 64 478 13.3891%
ncRNA_UTR5 0 0 0% 2 18 11.1111%
nonframeshift_deletion 0 0 0% 458 8777 5.2182%
nonframeshift_insertion 0 0 0% 229 4784 4.7868%
nonsynonymous 41720 336233 12.4081% 0 0 0%
splice_acceptor 86 750 11.4667% 53 964 5.4979%
splice_donor 126 1076 11.71% 28 763 3.6697%
splice_donor_acceptor 0 0 0% 10 45 22.2222%
splice_UTR5 69 400 17.25% 9 106 8.4906%
splie_Others 0 0 0% 38 654 5.8104%
startloss 132 671 19.6721% 3 51 5.8824%
stopgain 425 4175 10.1796% 19 1252 1.5176%
stoploss 65 353 18.4136% 5 63 7.9365%
synonymous 94703 548813 17.256% 0 0 0%
upstream 160886 679592 23.6739% 12092 98760 12.2438%
upstream;downstream 13520 57451 23.5331% 1126 9367 12.0209%
UTR3 74496 361040 20.6337% 6770 61415 11.0234%
UTR5 22768 112990 20.1505% 1596 16935 9.4243%
UTR5;UTR3 524 2524 20.7607% 39 381 10.2362%
Total 9067021 39753026 22.8084% 708457 5848802 12.1129%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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