Yugan Black-bone chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Yugan Black-bone chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

Yugan Black-bone chicken is a very ancient breed wich originated in Yugan County of Jiangxi Province, China in 2,000 years ago. In 1980, it was listed by international organization as a endangered local chicken breed. They are also known as Yugan Black chickens, Nancheng Black chickens. They are mainly distributed in towns in Yugan County and Nancheng County, and surrounding cities or counties.

Characteristics

Yugan Black-bone chickens are a local breed used for both meat and egg. The feathers of Yugan Black-bone chicken are black. The beaks, combs, skins, flesh, bones, toes are dark black. The rooster's heads are high, the tail feathers are upturned. The comb types are single comb, dark purplish red in color. The hens start laying at about 156 days and lay about 160 eggs a year.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 173941 650455 26.7414% 16504 104154 15.8458%
exonic;splicing 31 188 16.4894% 0 0 0%
exonic_unknown 23 582 3.9519% 0 82 0%
frameshift_deletion 0 0 0% 1034 15977 6.4718%
frameshift_insertion 0 0 0% 928 13308 6.9732%
intergenic 3946800 15129055 26.0876% 337487 2238383 15.0773%
intronic 4939900 17735594 27.853% 442774 2641780 16.7604%
ncRNA_exonic 104271 400185 26.0557% 8129 54342 14.959%
ncRNA_exonic;splicing 70 231 30.303% 3 43 6.9767%
ncRNA_intronic 996017 3728327 26.7149% 90476 575920 15.7098%
ncRNA_splicing 589 2341 25.1602% 78 478 16.318%
ncRNA_UTR5 0 0 0% 2 18 11.1111%
nonframeshift_deletion 0 0 0% 620 8777 7.0639%
nonframeshift_insertion 0 0 0% 280 4784 5.8528%
nonsynonymous 47760 336233 14.2044% 0 0 0%
splice_acceptor 104 750 13.8667% 90 964 9.3361%
splice_donor 120 1076 11.1524% 42 763 5.5046%
splice_donor_acceptor 0 0 0% 19 45 42.2222%
splice_UTR5 84 400 21% 9 106 8.4906%
splie_Others 0 0 0% 39 654 5.9633%
startloss 164 671 24.4411% 9 51 17.6471%
stopgain 524 4175 12.5509% 22 1252 1.7572%
stoploss 83 353 23.5127% 5 63 7.9365%
synonymous 105510 548813 19.2251% 0 0 0%
upstream 179936 679592 26.4771% 15050 98760 15.239%
upstream;downstream 15350 57451 26.7184% 1360 9367 14.5191%
UTR3 88203 361040 24.4303% 8998 61415 14.6511%
UTR5 25902 112990 22.9242% 2122 16935 12.5303%
UTR5;UTR3 616 2524 24.4057% 61 381 16.0105%
Total 10625998 39753026 26.73% 926141 5848802 15.8347%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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