Xuefeng Black-bone chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Xuefeng Black-bone chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

Xuefeng Black-bone chickens are a local breed used for meat and eggs. They originated around Xuefeng Mountain in southwest of Hunan Province, China.

Characteristics

The bodies of Xishuangbanna Game chickens are tall. The feathers are white, black, red and other mixed. The neck is long. The beaks are short, with yellow, brown, gray or other colors. The faces are red. Most individuals are bean comb, and few are single comb. The combs and wattles are small and reddish. The skins are white or yellow. The shanks are yellow, gray, black, and white. They have no shank feathers and toe feathers. They have four toes.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 165523 650455 25.4473% 15415 104154 14.8002%
exonic;splicing 33 188 17.5532% 0 0 0%
exonic_unknown 29 582 4.9828% 0 82 0%
frameshift_deletion 0 0 0% 922 15977 5.7708%
frameshift_insertion 0 0 0% 806 13308 6.0565%
intergenic 3778764 15129055 24.9769% 315306 2238383 14.0863%
intronic 4723804 17735594 26.6346% 415153 2641780 15.7149%
ncRNA_exonic 99153 400185 24.7768% 7696 54342 14.1622%
ncRNA_exonic;splicing 73 231 31.6017% 5 43 11.6279%
ncRNA_intronic 949295 3728327 25.4617% 84369 575920 14.6494%
ncRNA_splicing 596 2341 25.4592% 79 478 16.5272%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 508 8777 5.7879%
nonframeshift_insertion 0 0 0% 239 4784 4.9958%
nonsynonymous 43588 336233 12.9636% 0 0 0%
splice_acceptor 92 750 12.2667% 86 964 8.9212%
splice_donor 128 1076 11.8959% 30 763 3.9318%
splice_donor_acceptor 0 0 0% 19 45 42.2222%
splice_UTR5 73 400 18.25% 8 106 7.5472%
splie_Others 0 0 0% 42 654 6.422%
startloss 146 671 21.7586% 4 51 7.8431%
stopgain 460 4175 11.018% 22 1252 1.7572%
stoploss 74 353 20.9632% 6 63 9.5238%
synonymous 99886 548813 18.2004% 0 0 0%
upstream 170930 679592 25.1519% 13948 98760 14.1231%
upstream;downstream 14496 57451 25.2319% 1281 9367 13.6757%
UTR3 82743 361040 22.918% 8305 61415 13.5228%
UTR5 24185 112990 21.4045% 1897 16935 11.2017%
UTR5;UTR3 551 2524 21.8304% 47 381 12.336%
Total 10154622 39753026 25.5443% 866194 5848802 14.8098%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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