Xishuangbanna Game chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Xishuangbanna Game chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Fighting
Description

Xishuangbanna Game chickens are a local breed for cockfighting and ornamental. They originated in Xishuangbanna of Yunnan Province, China. It has a very long breeding history in China, which can be date back to 2,000 years ago.

Characteristics

The bodies of Xishuangbanna Game chickens are tall. The feathers are white, black, red and other mixed. The neck is long. The beaks are short, with yellow, brown, gray or other colors. The faces are red. Most individuals are bean comb, and few are single comb. The combs and wattles are small and reddish. The skins are white or yellow. The shanks are yellow, gray, black, and white. They have no shank feathers and toe feathers. They have four toes. The hens start laying at 180-210 days and lay 100-120 eggs a year.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 172321 650455 26.4924% 16030 104154 15.3907%
exonic;splicing 36 188 19.1489% 0 0 0%
exonic_unknown 49 582 8.4192% 0 82 0%
frameshift_deletion 0 0 0% 852 15977 5.3327%
frameshift_insertion 0 0 0% 726 13308 5.4554%
intergenic 3961203 15129055 26.1828% 334121 2238383 14.9269%
intronic 4960917 17735594 27.9715% 440319 2641780 16.6675%
ncRNA_exonic 103811 400185 25.9408% 8119 54342 14.9406%
ncRNA_exonic;splicing 64 231 27.7056% 5 43 11.6279%
ncRNA_intronic 994658 3728327 26.6784% 89484 575920 15.5376%
ncRNA_splicing 610 2341 26.0572% 75 478 15.6904%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 491 8777 5.5942%
nonframeshift_insertion 0 0 0% 230 4784 4.8077%
nonsynonymous 44337 336233 13.1864% 0 0 0%
splice_acceptor 105 750 14% 81 964 8.4025%
splice_donor 130 1076 12.0818% 38 763 4.9803%
splice_donor_acceptor 0 0 0% 17 45 37.7778%
splice_UTR5 80 400 20% 9 106 8.4906%
splie_Others 0 0 0% 37 654 5.6575%
startloss 146 671 21.7586% 3 51 5.8824%
stopgain 444 4175 10.6347% 17 1252 1.3578%
stoploss 77 353 21.813% 8 63 12.6984%
synonymous 105267 548813 19.1809% 0 0 0%
upstream 174099 679592 25.6182% 13935 98760 14.11%
upstream;downstream 14341 57451 24.9621% 1244 9367 13.2807%
UTR3 86798 361040 24.0411% 8607 61415 14.0145%
UTR5 23574 112990 20.8638% 1714 16935 10.1211%
UTR5;UTR3 551 2524 21.8304% 57 381 14.9606%
Total 10643618 39753026 26.7744% 916220 5848802 15.6651%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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