Wuliangshan Black-bone chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Wuliangshan Black-bone chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

Wuliangshan Black-bone chicken is a ancient local breed used for meat and eggs. Wuliangshan Black-bone chickens originated in Jingdong County, Yunnan Province, China.

Characteristics

Wuliangshan Black-bone chicken have yellow, black or white feathers. The beaks, shanks, toes are blue. They have single comb. Their shank and toe are feathered. The skin is black. The hens start laying at 180-200 days and lay 90-130 eggs a year.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 170100 650455 26.1509% 15868 104154 15.2351%
exonic;splicing 29 188 15.4255% 0 0 0%
exonic_unknown 41 582 7.0447% 0 82 0%
frameshift_deletion 0 0 0% 992 15977 6.2089%
frameshift_insertion 0 0 0% 874 13308 6.5675%
intergenic 3890218 15129055 25.7136% 326170 2238383 14.5717%
intronic 4835097 17735594 27.2621% 426638 2641780 16.1496%
ncRNA_exonic 101534 400185 25.3718% 7857 54342 14.4584%
ncRNA_exonic;splicing 79 231 34.1991% 5 43 11.6279%
ncRNA_intronic 965560 3728327 25.8979% 85617 575920 14.8661%
ncRNA_splicing 606 2341 25.8864% 74 478 15.4812%
ncRNA_UTR5 0 0 0% 2 18 11.1111%
nonframeshift_deletion 0 0 0% 541 8777 6.1638%
nonframeshift_insertion 0 0 0% 252 4784 5.2676%
nonsynonymous 44747 336233 13.3083% 0 0 0%
splice_acceptor 104 750 13.8667% 90 964 9.3361%
splice_donor 134 1076 12.4535% 28 763 3.6697%
splice_donor_acceptor 0 0 0% 22 45 48.8889%
splice_UTR5 87 400 21.75% 6 106 5.6604%
splie_Others 0 0 0% 36 654 5.5046%
startloss 151 671 22.5037% 7 51 13.7255%
stopgain 474 4175 11.3533% 26 1252 2.0767%
stoploss 88 353 24.9292% 7 63 11.1111%
synonymous 102336 548813 18.6468% 0 0 0%
upstream 174564 679592 25.6866% 14559 98760 14.7418%
upstream;downstream 14735 57451 25.6479% 1369 9367 14.6151%
UTR3 84738 361040 23.4705% 8516 61415 13.8663%
UTR5 24776 112990 21.9276% 2047 16935 12.0874%
UTR5;UTR3 581 2524 23.019% 53 381 13.9108%
Total 10410779 39753026 26.1886% 891656 5848802 15.2451%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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