Wenchang chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Wenchang chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Broiler
Description

Wenchang chicken is a local breed primarily used for meat. Wenchang chickens are native to Wenchang City, Hainan Province, China. This breed has a long breeding history which can be date bacck to 1,600-1,700.

Characteristics

The feathers of Wenchang chickens are yellow, white or black. They have small heads, short curved yellow beaks. They are single comb. The combs and wattles are red. The skins are white or pale yellow. The shanks are yellow.The rooster feathers are jujube red, the tail feathers are black with dark green luster. Hen's feathers are more tawny, the chest feathers are white. The hens start laying at 120-126 days and lay about 120 eggs a year.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 163980 650455 25.21% 14566 104154 13.9851%
exonic;splicing 32 188 17.0213% 0 0 0%
exonic_unknown 19 582 3.2646% 0 82 0%
frameshift_deletion 0 0 0% 863 15977 5.4015%
frameshift_insertion 0 0 0% 707 13308 5.3126%
intergenic 3715660 15129055 24.5598% 297866 2238383 13.3072%
intronic 4680631 17735594 26.3912% 400569 2641780 15.1628%
ncRNA_exonic 98196 400185 24.5377% 7334 54342 13.496%
ncRNA_exonic;splicing 64 231 27.7056% 4 43 9.3023%
ncRNA_intronic 936869 3728327 25.1284% 80100 575920 13.9082%
ncRNA_splicing 582 2341 24.8612% 75 478 15.6904%
ncRNA_UTR5 0 0 0% 2 18 11.1111%
nonframeshift_deletion 0 0 0% 479 8777 5.4574%
nonframeshift_insertion 0 0 0% 227 4784 4.745%
nonsynonymous 43040 336233 12.8006% 0 0 0%
splice_acceptor 98 750 13.0667% 81 964 8.4025%
splice_donor 117 1076 10.8736% 28 763 3.6697%
splice_donor_acceptor 0 0 0% 15 45 33.3333%
splice_UTR5 77 400 19.25% 9 106 8.4906%
splie_Others 0 0 0% 45 654 6.8807%
startloss 149 671 22.2057% 3 51 5.8824%
stopgain 433 4175 10.3713% 21 1252 1.6773%
stoploss 83 353 23.5127% 8 63 12.6984%
synonymous 99932 548813 18.2088% 0 0 0%
upstream 170187 679592 25.0425% 13440 98760 13.6087%
upstream;downstream 14326 57451 24.936% 1227 9367 13.0992%
UTR3 82501 361040 22.8509% 7878 61415 12.8275%
UTR5 23812 112990 21.0744% 1716 16935 10.1329%
UTR5;UTR3 546 2524 21.6323% 60 381 15.748%
Total 10031334 39753026 25.2341% 827323 5848802 14.1452%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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