Tibetan chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Tibetan chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

Tibetan chickens are a local breed used for meat and eggs. It is a unique poultry resource which adapted plateau environment.

Characteristics

Tibetan chicken bodies are small. They are active, nervousness and aggressive. The primary feathers and tail feathers are strong, they are good at flying. The beaks are black and a few are fleshy or yellow. The combs are red single comb, a few are bean comb with comb feathers. The wattles are red. The shanks are black, followed by fleshcolor, a few have shank feathers. The skins are white or light yellow, a few are black.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 177336 650455 27.2634% 14646 104154 14.0619%
exonic;splicing 30 188 15.9574% 0 0 0%
exonic_unknown 41 582 7.0447% 0 82 0%
frameshift_deletion 0 0 0% 668 15977 4.181%
frameshift_insertion 0 0 0% 593 13308 4.456%
intergenic 4029647 15129055 26.6352% 301233 2238383 13.4576%
intronic 5084032 17735594 28.6657% 409238 2641780 15.491%
ncRNA_exonic 106506 400185 26.6142% 7506 54342 13.8125%
ncRNA_exonic;splicing 76 231 32.9004% 4 43 9.3023%
ncRNA_intronic 1006474 3728327 26.9953% 80682 575920 14.0092%
ncRNA_splicing 628 2341 26.8261% 79 478 16.5272%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 440 8777 5.0131%
nonframeshift_insertion 0 0 0% 212 4784 4.4314%
nonsynonymous 45267 336233 13.463% 0 0 0%
splice_acceptor 105 750 14% 64 964 6.639%
splice_donor 127 1076 11.803% 33 763 4.325%
splice_donor_acceptor 0 0 0% 11 45 24.4444%
splice_UTR5 75 400 18.75% 6 106 5.6604%
splie_Others 0 0 0% 42 654 6.422%
startloss 160 671 23.845% 5 51 9.8039%
stopgain 441 4175 10.5629% 16 1252 1.278%
stoploss 81 353 22.9462% 7 63 11.1111%
synonymous 108152 548813 19.7065% 0 0 0%
upstream 182724 679592 26.8873% 13189 98760 13.3546%
upstream;downstream 15001 57451 26.1109% 1180 9367 12.5974%
UTR3 88258 361040 24.4455% 7942 61415 12.9317%
UTR5 25327 112990 22.4153% 1660 16935 9.8022%
UTR5;UTR3 590 2524 23.3756% 58 381 15.2231%
Total 10871078 39753026 27.3465% 839515 5848802 14.3536%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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