Spanish
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Spanish
Species taxonomy Gallus gallus domesticus
Classification Village
Region Spain
Purpose Unclear
Description

The Spanish (standardised as White Faced Black Spanish in North America) is a breed of domestic chicken which originated in Spain. It is one of the oldest Mediterranean breeds of poultry and the first foreign breed to arrive in America. Despite its name, the origin is unclear and although many modern texts say it came from Spain. It was largely bred to its present type in Great Britain in the eighteenth century. It is an older breed than the Minorca.

Characteristics

The Spanish chickens have glossy black plumage and are closely related to the Minorca and Castellana Negra breeds. Their most distinguishing feature are their white, low-hanging ear-lobes, which are actually overdeveloped. They have a single comb, four toes, and no crest.The Spanish is very slow to mature, with the distinctive white face becoming more apparent after the first year of the bird. Hens are non-sitters, and lay 160-180 white eggs per year, with a weight of about 80 g. The average weights are 3.2 kg for roosters and 2.7 kg for hens.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 95138 650455 14.6264% 8926 104154 8.57%
exonic;splicing 18 188 9.5745% 0 0 0%
exonic_unknown 2 582 0.3436% 0 82 0%
frameshift_deletion 0 0 0% 541 15977 3.3861%
frameshift_insertion 0 0 0% 487 13308 3.6595%
intergenic 2183872 15129055 14.435% 185451 2238383 8.285%
intronic 2700799 17735594 15.2281% 238591 2641780 9.0314%
ncRNA_exonic 56423 400185 14.0992% 4366 54342 8.0343%
ncRNA_exonic;splicing 31 231 13.4199% 5 43 11.6279%
ncRNA_intronic 545837 3728327 14.6403% 49028 575920 8.513%
ncRNA_splicing 325 2341 13.883% 41 478 8.5774%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 285 8777 3.2471%
nonframeshift_insertion 0 0 0% 151 4784 3.1564%
nonsynonymous 24674 336233 7.3384% 0 0 0%
splice_acceptor 47 750 6.2667% 54 964 5.6017%
splice_donor 58 1076 5.3903% 24 763 3.1455%
splice_donor_acceptor 0 0 0% 15 45 33.3333%
splice_UTR5 48 400 12% 4 106 3.7736%
splie_Others 0 0 0% 27 654 4.1284%
startloss 80 671 11.9225% 1 51 1.9608%
stopgain 270 4175 6.4671% 12 1252 0.9585%
stoploss 49 353 13.881% 3 63 4.7619%
synonymous 57476 548813 10.4728% 0 0 0%
upstream 98335 679592 14.4697% 7987 98760 8.0873%
upstream;downstream 8022 57451 13.9632% 685 9367 7.3129%
UTR3 46864 361040 12.9803% 4922 61415 8.0143%
UTR5 13601 112990 12.0373% 1035 16935 6.1116%
UTR5;UTR3 340 2524 13.4707% 39 381 10.2362%
Total 5832309 39753026 14.6714% 502681 5848802 8.5946%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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