Silkies
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Silkies
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

The Silkie (Silky) is one of the ancient domestic chickens in China, which is also called Taihe Silkie, Wushan chicken, Bairong Silkie or Zhusi chicken. Although it has been mentioned by authorities for several hundred years, its origins are still disputed. The breed is a double-purpose native breed which origin and central breeding area are Taihe County of Jiangxi Province and some Coastal area of south Fujian, and now it has been spread all over the world and is often used as an ornamental chicken.

Characteristics

The breed is characterized by black skin and bones, walnut comb, blue earlobes, five toes, and fluffy plumage. Silkie hens have persistent broodiness to provide reliable broody for the eggs of other large fowls or bantams, although they are poor layers of cream or tinted eggs (100 eggs per year max). On avarage, the weights for rooster and hen are 1.8 kg and 1.36 kg, respectively.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 159103 650455 24.4603% 13791 104154 13.241%
exonic;splicing 31 188 16.4894% 0 0 0%
exonic_unknown 24 582 4.1237% 0 82 0%
frameshift_deletion 0 0 0% 567 15977 3.5489%
frameshift_insertion 0 0 0% 516 13308 3.8774%
intergenic 3694996 15129055 24.4232% 291693 2238383 13.0314%
intronic 4593994 17735594 25.9027% 382960 2641780 14.4963%
ncRNA_exonic 96953 400185 24.227% 7135 54342 13.1298%
ncRNA_exonic;splicing 64 231 27.7056% 4 43 9.3023%
ncRNA_intronic 937231 3728327 25.1381% 78628 575920 13.6526%
ncRNA_splicing 562 2341 24.0068% 69 478 14.4351%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 403 8777 4.5915%
nonframeshift_insertion 0 0 0% 168 4784 3.5117%
nonsynonymous 39949 336233 11.8813% 0 0 0%
splice_acceptor 92 750 12.2667% 67 964 6.9502%
splice_donor 104 1076 9.6654% 25 763 3.2765%
splice_donor_acceptor 0 0 0% 14 45 31.1111%
splice_UTR5 66 400 16.5% 9 106 8.4906%
splie_Others 0 0 0% 27 654 4.1284%
startloss 126 671 18.7779% 2 51 3.9216%
stopgain 410 4175 9.8204% 15 1252 1.1981%
stoploss 73 353 20.6799% 6 63 9.5238%
synonymous 93057 548813 16.956% 0 0 0%
upstream 154700 679592 22.7637% 11412 98760 11.5553%
upstream;downstream 12393 57451 21.5714% 956 9367 10.206%
UTR3 80105 361040 22.1873% 7501 61415 12.2136%
UTR5 19786 112990 17.5113% 1242 16935 7.3339%
UTR5;UTR3 499 2524 19.7702% 41 381 10.7612%
Total 9884318 39753026 24.8643% 797252 5848802 13.631%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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