Shimian Caoke chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Shimian Caoke chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

The Shimian caoke chicken is a local breed used for meat and eggs. The Shimian caoke chickens originated from Caoke Tibetan Town, Shimian County of Sichuan Province, China. It has a long breeding history more than 300 years.

Characteristics

The Shimian caoke chickens are large in body size, thick and long in necks. They can be classified into two strains: "cyan foot with red feather" and "black bone with black feather". The beaks are straight and short with black or gray color. The comb types are single comb. On avarage, the weights for rooster and hen are 3.5 kg and 2.2 kg, respectively.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 92605 650455 14.237% 7643 104154 7.3382%
exonic;splicing 21 188 11.1702% 0 0 0%
exonic_unknown 26 582 4.4674% 0 82 0%
frameshift_deletion 0 0 0% 538 15977 3.3673%
frameshift_insertion 0 0 0% 455 13308 3.419%
intergenic 1980271 15129055 13.0892% 143693 2238383 6.4195%
intronic 2463404 17735594 13.8896% 189351 2641780 7.1676%
ncRNA_exonic 53266 400185 13.3103% 3657 54342 6.7296%
ncRNA_exonic;splicing 39 231 16.8831% 2 43 4.6512%
ncRNA_intronic 475560 3728327 12.7553% 36303 575920 6.3035%
ncRNA_splicing 317 2341 13.5412% 34 478 7.113%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 280 8777 3.1902%
nonframeshift_insertion 0 0 0% 138 4784 2.8846%
nonsynonymous 26446 336233 7.8654% 0 0 0%
splice_acceptor 51 750 6.8% 41 964 4.2531%
splice_donor 86 1076 7.9926% 13 763 1.7038%
splice_donor_acceptor 0 0 0% 5 45 11.1111%
splice_UTR5 49 400 12.25% 4 106 3.7736%
splie_Others 0 0 0% 27 654 4.1284%
startloss 88 671 13.1148% 3 51 5.8824%
stopgain 270 4175 6.4671% 14 1252 1.1182%
stoploss 44 353 12.4646% 4 63 6.3492%
synonymous 61064 548813 11.1266% 0 0 0%
upstream 104962 679592 15.4449% 7862 98760 7.9607%
upstream;downstream 9266 57451 16.1285% 783 9367 8.3591%
UTR3 44770 361040 12.4003% 3889 61415 6.3323%
UTR5 15265 112990 13.51% 1114 16935 6.5781%
UTR5;UTR3 373 2524 14.7781% 36 381 9.4488%
Total 5328243 39753026 13.4034% 395890 5848802 6.7687%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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