Scots Grey
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Scots Grey
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region England
Purpose Dual
Description

The Scots Grey is a dual-purpose breed originated in Scotland, where it has been bred for more than two centuries. It was formerly known as the Scotch Grey and until about 1930 was popular in Scotland. It is on the "Native Poultry Breeds at Risk" list of the Rare Breeds Survival Trust.

Characteristics

The Scots Grey is a tall, upright chicken, with a single comb and bright red face and earlobes. Scots Grey hens are good layers of large white eggs (180 - 200 eggs per year), but they are non-sitters. It comes in a single color-variety: the plumage is barred, the ground color is steel-grey and the markings are larger in hens than in roosters. On avarage, the weights for rooster and hen are 3.2 kg and 2.25 kg, respectively.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 93241 650455 14.3347% 8854 104154 8.5009%
exonic;splicing 21 188 11.1702% 0 0 0%
exonic_unknown 0 582 0% 0 82 0%
frameshift_deletion 0 0 0% 499 15977 3.1232%
frameshift_insertion 0 0 0% 498 13308 3.7421%
intergenic 2128765 15129055 14.0707% 184663 2238383 8.2498%
intronic 2678429 17735594 15.102% 241195 2641780 9.13%
ncRNA_exonic 56872 400185 14.2114% 4482 54342 8.2478%
ncRNA_exonic;splicing 40 231 17.316% 4 43 9.3023%
ncRNA_intronic 547027 3728327 14.6722% 50203 575920 8.717%
ncRNA_splicing 346 2341 14.78% 48 478 10.0418%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 292 8777 3.3269%
nonframeshift_insertion 0 0 0% 142 4784 2.9682%
nonsynonymous 23842 336233 7.0909% 0 0 0%
splice_acceptor 52 750 6.9333% 49 964 5.083%
splice_donor 58 1076 5.3903% 15 763 1.9659%
splice_donor_acceptor 0 0 0% 14 45 31.1111%
splice_UTR5 48 400 12% 8 106 7.5472%
splie_Others 0 0 0% 29 654 4.4343%
startloss 89 671 13.2638% 4 51 7.8431%
stopgain 259 4175 6.2036% 11 1252 0.8786%
stoploss 47 353 13.3144% 5 63 7.9365%
synonymous 55767 548813 10.1614% 0 0 0%
upstream 96398 679592 14.1847% 8071 98760 8.1723%
upstream;downstream 8182 57451 14.2417% 722 9367 7.7079%
UTR3 46095 361040 12.7673% 4936 61415 8.0371%
UTR5 13598 112990 12.0347% 1066 16935 6.2947%
UTR5;UTR3 283 2524 11.2124% 21 381 5.5118%
Total 5749459 39753026 14.4629% 505832 5848802 8.6485%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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