Pita Pinta Asturiana
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Pita Pinta Asturiana
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region Spain
Purpose Dual
Description

The Pita Pinta Asturiana (literally means "the Asturian Painted Chicken"), is the only breed indigenous to the principality of Asturias, in north-western Spain. This breed shares origins with other Spanish breeds such as the Euskal Oiloa.

Characteristics

Pita Pinta chickens are medium to large-sized compact chickens, with single combs, and red earlobes. They have yellow legs with black spots, and come in four color-varieties. The most commonly seen color-varieties of the Pita Pinta are the mottled-black and the mottled-brown. Pita Pinta hens are good layers of burnt-cream eggs (around 160 eggs per year of weight 60 - 65 g per each), but they rarely go broody. The roosters weigh 4 - 4.5 kg; and the hens weigh female: 2.5 - 3 kg.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 162259 650455 24.9455% 15012 104154 14.4133%
exonic;splicing 34 188 18.0851% 0 0 0%
exonic_unknown 39 582 6.701% 0 82 0%
frameshift_deletion 0 0 0% 917 15977 5.7395%
frameshift_insertion 0 0 0% 766 13308 5.7559%
intergenic 3698901 15129055 24.449% 312073 2238383 13.9419%
intronic 4611551 17735594 26.0017% 406366 2641780 15.3823%
ncRNA_exonic 97167 400185 24.2805% 7514 54342 13.8272%
ncRNA_exonic;splicing 75 231 32.4675% 7 43 16.2791%
ncRNA_intronic 933511 3728327 25.0383% 83296 575920 14.4631%
ncRNA_splicing 577 2341 24.6476% 76 478 15.8996%
ncRNA_UTR5 0 0 0% 0 18 0%
nonframeshift_deletion 0 0 0% 557 8777 6.3461%
nonframeshift_insertion 0 0 0% 268 4784 5.602%
nonsynonymous 43628 336233 12.9755% 0 0 0%
splice_acceptor 98 750 13.0667% 77 964 7.9876%
splice_donor 125 1076 11.6171% 36 763 4.7182%
splice_donor_acceptor 0 0 0% 18 45 40%
splice_UTR5 80 400 20% 7 106 6.6038%
splie_Others 0 0 0% 46 654 7.0336%
startloss 137 671 20.4173% 7 51 13.7255%
stopgain 466 4175 11.1617% 26 1252 2.0767%
stoploss 84 353 23.796% 7 63 11.1111%
synonymous 98518 548813 17.9511% 0 0 0%
upstream 167436 679592 24.6377% 13427 98760 13.5956%
upstream;downstream 14168 57451 24.661% 1232 9367 13.1526%
UTR3 81249 361040 22.5042% 8219 61415 13.3827%
UTR5 23850 112990 21.1081% 1792 16935 10.5816%
UTR5;UTR3 559 2524 22.1474% 54 381 14.1732%
Total 9934512 39753026 24.9906% 851800 5848802 14.5637%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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