Malay
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Malay
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region Southeast Asia
Purpose Fighting
Description

The Malay is an old breed with Asian background from the remote East. It has been bred long ago, in parts of Northern India, Indonesia and Malaysia. The Malay is known to be the tallest chicken in the world, as it may stand over 90 cm high. Malsy chickens first came to England around 1830 from Asia, originally for cockfighting purposes due to their gamey nature. As cockfighting has been outlawed in Europe and the USA, the bird is mainly used in poultry shows instead in the mean time.

Characteristics

Malays are very large birds with upright stance, long legs and neck that make them look larger. They are energetic birds with a meaty body, though their meat is too tough and firm. Malays are available in a lot of color-varieties but the most common are; black, wheaten, spangled and white. The Malay has a pea comb, red earlobes, tiny wattles and bright-yellow legs. On avarage, the weights for rooster and hen are 5 kg and 4.1 kg, respectively.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 107348 650455 16.5035% 9501 104154 9.1221%
exonic;splicing 21 188 11.1702% 0 0 0%
exonic_unknown 2 582 0.3436% 0 82 0%
frameshift_deletion 0 0 0% 507 15977 3.1733%
frameshift_insertion 0 0 0% 454 13308 3.4115%
intergenic 2461591 15129055 16.2706% 201074 2238383 8.983%
intronic 3067813 17735594 17.2975% 263393 2641780 9.9703%
ncRNA_exonic 64469 400185 16.1098% 4944 54342 9.0979%
ncRNA_exonic;splicing 46 231 19.9134% 5 43 11.6279%
ncRNA_intronic 626304 3728327 16.7985% 54382 575920 9.4426%
ncRNA_splicing 396 2341 16.9158% 43 478 8.9958%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 270 8777 3.0762%
nonframeshift_insertion 0 0 0% 135 4784 2.8219%
nonsynonymous 27236 336233 8.1003% 0 0 0%
splice_acceptor 63 750 8.4% 46 964 4.7718%
splice_donor 72 1076 6.6914% 19 763 2.4902%
splice_donor_acceptor 0 0 0% 8 45 17.7778%
splice_UTR5 52 400 13% 3 106 2.8302%
splie_Others 0 0 0% 30 654 4.5872%
startloss 78 671 11.6244% 3 51 5.8824%
stopgain 269 4175 6.4431% 12 1252 0.9585%
stoploss 52 353 14.7309% 2 63 3.1746%
synonymous 64270 548813 11.7107% 0 0 0%
upstream 107132 679592 15.7642% 8231 98760 8.3343%
upstream;downstream 8893 57451 15.4793% 716 9367 7.6439%
UTR3 52658 361040 14.5851% 5247 61415 8.5435%
UTR5 14531 112990 12.8604% 995 16935 5.8754%
UTR5;UTR3 323 2524 12.7971% 34 381 8.9239%
Total 6603619 39753026 16.6116% 550055 5848802 9.4046%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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