Leghorn
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Leghorn
Species taxonomy Gallus gallus domesticus
Classification Commercial
Region Italy
Purpose Layer
Description

The Leghorn is a breed that originated in Tuscany of Central Italy. It was initially called as "Italians". Since 1865, the breed has been known as "Leghorn", that is, the traditional Anglicization of "Livorno". Livorno is the port city from which Leghorns were exported to North America for the first time. In Germany, Leghorns are called "Italieners". White Leghorns are commonly used as layer chickens in many countries of the world. It is quite often thought of as an American bird since it was refined and perfected into a stable breed in the United States before being shipped back to Europe.

Characteristics

Leghorns have white earlobes, bright-yellow legs, and large combs and wattles. The breed comes comes in two varieties; the single-combed and the less common rose-combed. A lot of color-varieties are also recognized for the Leghorn. The chicks are easy to rear; feather up quickly and are fast growers. The avarage weights at 7.5 lbs for males and 5-6 lbs for hens. The bantams weigh in at 1kg for males 0.9kg for hens. The Leghorn hen lays anywhere from 280 - 320 eggs (around 55g each) per year without going broody.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 130390 650455 20.046% 11859 104154 11.386%
exonic;splicing 26 188 13.8298% 0 0 0%
exonic_unknown 26 582 4.4674% 0 82 0%
frameshift_deletion 0 0 0% 630 15977 3.9432%
frameshift_insertion 0 0 0% 510 13308 3.8323%
intergenic 3006316 15129055 19.8711% 253971 2238383 11.3462%
intronic 3750226 17735594 21.1452% 330341 2641780 12.5045%
ncRNA_exonic 78388 400185 19.5879% 6058 54342 11.1479%
ncRNA_exonic;splicing 62 231 26.8398% 7 43 16.2791%
ncRNA_intronic 758474 3728327 20.3435% 67683 575920 11.7522%
ncRNA_splicing 469 2341 20.0342% 59 478 12.3431%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 362 8777 4.1244%
nonframeshift_insertion 0 0 0% 156 4784 3.2609%
nonsynonymous 32938 336233 9.7962% 0 0 0%
splice_acceptor 62 750 8.2667% 61 964 6.3278%
splice_donor 96 1076 8.9219% 25 763 3.2765%
splice_donor_acceptor 0 0 0% 17 45 37.7778%
splice_UTR5 65 400 16.25% 7 106 6.6038%
splie_Others 0 0 0% 35 654 5.3517%
startloss 116 671 17.2876% 1 51 1.9608%
stopgain 341 4175 8.1677% 18 1252 1.4377%
stoploss 66 353 18.6969% 6 63 9.5238%
synonymous 77138 548813 14.0554% 0 0 0%
upstream 131266 679592 19.3154% 10321 98760 10.4506%
upstream;downstream 10768 57451 18.7429% 899 9367 9.5975%
UTR3 64674 361040 17.9133% 6556 61415 10.6749%
UTR5 17626 112990 15.5996% 1217 16935 7.1863%
UTR5;UTR3 424 2524 16.7987% 40 381 10.4987%
Total 8059957 39753026 20.2751% 690840 5848802 11.8116%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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