Kedu Hitam chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Kedu Hitam chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region Indonesia
Purpose Fancy
Description

Kedu Hitam is one of the oldest breeds in Indonesia. It is used primarily for cockfighting and recently for ornamental purposes and as sources of eggs. This breed is adapted to roughage diet and to the hot and humid climate. The Kedu Hitam chicken was brought to India, Europe, and America in the eighteenth century. Kedu Hitam was crossed with other unknown breeds to develop Black Java, which was admitted to the American Standard in 1910.

Characteristics

The feather is black and comb is red.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 158138 650455 24.3119% 14064 104154 13.5031%
exonic;splicing 32 188 17.0213% 0 0 0%
exonic_unknown 18 582 3.0928% 0 82 0%
frameshift_deletion 0 0 0% 706 15977 4.4189%
frameshift_insertion 0 0 0% 608 13308 4.5687%
intergenic 3582857 15129055 23.682% 288476 2238383 12.8877%
intronic 4507390 17735594 25.4144% 384803 2641780 14.5661%
ncRNA_exonic 94013 400185 23.4924% 7015 54342 12.909%
ncRNA_exonic;splicing 65 231 28.1385% 2 43 4.6512%
ncRNA_intronic 895237 3728327 24.0118% 77005 575920 13.3708%
ncRNA_splicing 575 2341 24.5622% 68 478 14.2259%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 436 8777 4.9675%
nonframeshift_insertion 0 0 0% 199 4784 4.1597%
nonsynonymous 40981 336233 12.1883% 0 0 0%
splice_acceptor 89 750 11.8667% 67 964 6.9502%
splice_donor 113 1076 10.5019% 26 763 3.4076%
splice_donor_acceptor 0 0 0% 12 45 26.6667%
splice_UTR5 67 400 16.75% 10 106 9.434%
splie_Others 0 0 0% 36 654 5.5046%
startloss 124 671 18.4799% 3 51 5.8824%
stopgain 424 4175 10.1557% 19 1252 1.5176%
stoploss 77 353 21.813% 6 63 9.5238%
synonymous 95759 548813 17.4484% 0 0 0%
upstream 163106 679592 24.0006% 12477 98760 12.6337%
upstream;downstream 13786 57451 23.9961% 1146 9367 12.2344%
UTR3 79396 361040 21.9909% 7589 61415 12.3569%
UTR5 22723 112990 20.1106% 1696 16935 10.0148%
UTR5;UTR3 538 2524 21.3154% 42 381 11.0236%
Total 9655508 39753026 24.2887% 796512 5848802 13.6184%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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