Jiuyuan Black-bone chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Jiuyuan Black-bone chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

Jiuyuan Black-bone chickens are local breed used for meat and eggs. Jiuyuan Black-bone chickens originated form Jiuyuan and Baiyang and other townships in Wanyuan City, Sichuan Province, China.

Characteristics

The bodies of Jiuyuan Black-bone chickens are larger, the beaks are black, the comb types are single comb or bean comb. The combs and wattles are red or purplish black. The skins are white or black. The shanks are black, and a few individuals have shank feathers.The rooster feathers are black or red, the neck feathers, saddle feathers and sickle feathers are black with red border and shanky. The hen feathers are black with emerald green luster, a few hen neck feathers are black with red border.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 161841 650455 24.8812% 14061 104154 13.5002%
exonic;splicing 31 188 16.4894% 0 0 0%
exonic_unknown 30 582 5.1546% 0 82 0%
frameshift_deletion 0 0 0% 918 15977 5.7458%
frameshift_insertion 0 0 0% 771 13308 5.7935%
intergenic 3596284 15129055 23.7707% 279439 2238383 12.484%
intronic 4501812 17735594 25.3829% 373816 2641780 14.1502%
ncRNA_exonic 96339 400185 24.0736% 6884 54342 12.6679%
ncRNA_exonic;splicing 69 231 29.8701% 3 43 6.9767%
ncRNA_intronic 893712 3728327 23.9709% 73634 575920 12.7855%
ncRNA_splicing 545 2341 23.2806% 70 478 14.6444%
ncRNA_UTR5 0 0 0% 0 18 0%
nonframeshift_deletion 0 0 0% 514 8777 5.8562%
nonframeshift_insertion 0 0 0% 247 4784 5.163%
nonsynonymous 45042 336233 13.3961% 0 0 0%
splice_acceptor 91 750 12.1333% 68 964 7.0539%
splice_donor 118 1076 10.9665% 32 763 4.194%
splice_donor_acceptor 0 0 0% 17 45 37.7778%
splice_UTR5 77 400 19.25% 5 106 4.717%
splie_Others 0 0 0% 35 654 5.3517%
startloss 136 671 20.2683% 7 51 13.7255%
stopgain 473 4175 11.3293% 25 1252 1.9968%
stoploss 77 353 21.813% 7 63 11.1111%
synonymous 101770 548813 18.5437% 0 0 0%
upstream 170944 679592 25.1539% 13252 98760 13.4184%
upstream;downstream 14542 57451 25.312% 1198 9367 12.7896%
UTR3 81022 361040 22.4413% 7473 61415 12.168%
UTR5 24299 112990 21.5054% 1797 16935 10.6112%
UTR5;UTR3 556 2524 22.0285% 49 381 12.8609%
Total 9689810 39753026 24.375% 774322 5848802 13.239%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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