Jiangshan Black-bone chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Jiangshan Black-bone chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

Jiangshan black-bone chicken, commonly known as "Jiangshan white feather black-bone chicken", is a dual purpose local breed. The origin and central distribution area of Jiangshan black-bone chicken are Tanshi, Qinghu, Shuangta, Hushan and other towns in Jiangshan City, Zhejiang Province, China. It is a ancient local breed with more than 1,700 years of history.

Characteristics

The whole body feathers of Jiangshan black-bone chicken are white, The beaks, tongues, shanks, toes, meat, bones and skins are black. The comb types are erect single comb. The combs and wattles are black and the irises are brown. The shanks are feathered. The hens start laying at about 150 days and lay 127 eggs a year.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 152480 650455 23.4421% 14150 104154 13.5857%
exonic;splicing 29 188 15.4255% 0 0 0%
exonic_unknown 25 582 4.2955% 0 82 0%
frameshift_deletion 0 0 0% 831 15977 5.2012%
frameshift_insertion 0 0 0% 748 13308 5.6207%
intergenic 3470370 15129055 22.9384% 289613 2238383 12.9385%
intronic 4345025 17735594 24.4989% 380658 2641780 14.4091%
ncRNA_exonic 91167 400185 22.7812% 7057 54342 12.9863%
ncRNA_exonic;splicing 57 231 24.6753% 3 43 6.9767%
ncRNA_intronic 876441 3728327 23.5076% 77744 575920 13.4991%
ncRNA_splicing 532 2341 22.7253% 65 478 13.5983%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 473 8777 5.3891%
nonframeshift_insertion 0 0 0% 220 4784 4.5987%
nonsynonymous 40452 336233 12.0309% 0 0 0%
splice_acceptor 92 750 12.2667% 87 964 9.0249%
splice_donor 113 1076 10.5019% 25 763 3.2765%
splice_donor_acceptor 0 0 0% 19 45 42.2222%
splice_UTR5 72 400 18% 7 106 6.6038%
splie_Others 0 0 0% 32 654 4.893%
startloss 132 671 19.6721% 6 51 11.7647%
stopgain 436 4175 10.4431% 18 1252 1.4377%
stoploss 76 353 21.5297% 5 63 7.9365%
synonymous 91567 548813 16.6846% 0 0 0%
upstream 156962 679592 23.0965% 12827 98760 12.9881%
upstream;downstream 13101 57451 22.8038% 1187 9367 12.6721%
UTR3 76157 361040 21.0938% 7526 61415 12.2543%
UTR5 22020 112990 19.4885% 1728 16935 10.2037%
UTR5;UTR3 528 2524 20.9192% 51 381 13.3858%
Total 9337834 39753026 23.4896% 795081 5848802 13.5939%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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