Henan Game chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Henan Game chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Fighting
Description

Henan Game chicken also known as the Zhongyuan Game chicken, is an ornamental local breed used for cockfighting. The origin and distribution area of Henan Game chickens are Kaifeng City, Henan Province, China.

Characteristics

Henan Game chickens are tall and large in size. The feathers are black, red, purper or white. The skin and shank are yellow. They have small pea comb. The roosters weight about 3.5 kg and hens weight about 2.9 kg. They start laying at about 246 days and lay about 100 eggs a year.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 91517 650455 14.0697% 7820 104154 7.5081%
exonic;splicing 15 188 7.9787% 0 0 0%
exonic_unknown 12 582 2.0619% 0 82 0%
frameshift_deletion 0 0 0% 435 15977 2.7227%
frameshift_insertion 0 0 0% 361 13308 2.7127%
intergenic 2095036 15129055 13.8478% 161866 2238383 7.2314%
intronic 2646423 17735594 14.9215% 215226 2641780 8.147%
ncRNA_exonic 54895 400185 13.7174% 3971 54342 7.3074%
ncRNA_exonic;splicing 34 231 14.7186% 3 43 6.9767%
ncRNA_intronic 533077 3728327 14.298% 43552 575920 7.5622%
ncRNA_splicing 283 2341 12.0889% 25 478 5.2301%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 259 8777 2.9509%
nonframeshift_insertion 0 0 0% 129 4784 2.6965%
nonsynonymous 23782 336233 7.0731% 0 0 0%
splice_acceptor 53 750 7.0667% 36 964 3.7344%
splice_donor 52 1076 4.8327% 15 763 1.9659%
splice_donor_acceptor 0 0 0% 7 45 15.5556%
splice_UTR5 36 400 9% 3 106 2.8302%
splie_Others 0 0 0% 22 654 3.3639%
startloss 79 671 11.7735% 3 51 5.8824%
stopgain 247 4175 5.9162% 12 1252 0.9585%
stoploss 39 353 11.0482% 3 63 4.7619%
synonymous 54931 548813 10.0091% 0 0 0%
upstream 92868 679592 13.6653% 6875 98760 6.9613%
upstream;downstream 7550 57451 13.1416% 612 9367 6.5336%
UTR3 45745 361040 12.6703% 4152 61415 6.7606%
UTR5 12709 112990 11.2479% 833 16935 4.9188%
UTR5;UTR3 276 2524 10.935% 20 381 5.2493%
Total 5659659 39753026 14.2371% 446241 5848802 7.6296%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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