Gushi chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Gushi chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

Gushi chickens are a local breed used for meat and eggs. The origin of Gushi chickens is Gushi County of Henan Province, China. They are distributed in 28 provinces across the country.

Characteristics

Gushi chicken bodies are medium. Their beaks are short and curved. They have erect single comb, a few are bean comb. The combs, wattles, and earlobes are red. The skins are mostly white and a few are black. The shanks and toes are blue.The rooster feather color is deep red or yellow, the comb feathers are bright and beautiful, the tail shape is mainly finger-like tail, the sickle feathers are more with black. The hen feathers are yellow.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 87739 650455 13.4889% 7639 104154 7.3343%
exonic;splicing 12 188 6.383% 0 0 0%
exonic_unknown 18 582 3.0928% 0 82 0%
frameshift_deletion 0 0 0% 207 15977 1.2956%
frameshift_insertion 0 0 0% 183 13308 1.3751%
intergenic 2266385 15129055 14.9803% 177366 2238383 7.9238%
intronic 2841398 17735594 16.0209% 236689 2641780 8.9595%
ncRNA_exonic 58323 400185 14.574% 4328 54342 7.9644%
ncRNA_exonic;splicing 37 231 16.0173% 3 43 6.9767%
ncRNA_intronic 604845 3728327 16.223% 50341 575920 8.741%
ncRNA_splicing 344 2341 14.6946% 38 478 7.9498%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 179 8777 2.0394%
nonframeshift_insertion 0 0 0% 64 4784 1.3378%
nonsynonymous 17459 336233 5.1925% 0 0 0%
splice_acceptor 44 750 5.8667% 32 964 3.3195%
splice_donor 57 1076 5.2974% 11 763 1.4417%
splice_donor_acceptor 0 0 0% 10 45 22.2222%
splice_UTR5 35 400 8.75% 2 106 1.8868%
splie_Others 0 0 0% 11 654 1.682%
startloss 49 671 7.3025% 0 51 0%
stopgain 150 4175 3.5928% 6 1252 0.4792%
stoploss 33 353 9.3484% 2 63 3.1746%
synonymous 44219 548813 8.0572% 0 0 0%
upstream 68591 679592 10.093% 5080 98760 5.1438%
upstream;downstream 3973 57451 6.9155% 319 9367 3.4056%
UTR3 44793 361040 12.4067% 4204 61415 6.8452%
UTR5 7357 112990 6.5112% 471 16935 2.7812%
UTR5;UTR3 164 2524 6.4976% 17 381 4.4619%
Total 6046025 39753026 15.209% 487203 5848802 8.33%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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