Guangxi Black-bone chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Guangxi Black-bone chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

The Guangxi Black-bone chicken is also known as "Donglan Black-bone chicken" in Donglan County and "Lingyun Black-bone chicken" in Lingyun County. The origin of Guangxi Black-bone chicken is Donglan County and Lingyun County of Guangxi Zhuang Autonomous Region, China.

Characteristics

The Guangxi Black-bone chicken is medium in body size. The chicken in Donglan County is dominated by black feathers, while the chicken in Lingyun County is dominated by yellow feathers.The Beak is black. The chickens have single comb, the wattles, and earlobes are black. Ear feathers are black or light yellow. Skin and muscles are black. Most of them have four toes and a few have five toes. Shanks are black or bluish gray, with a few shank feathers.The rooster feather color is black and linen. The hen feather color is black and linen, a few are yellow or white.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 186506 650455 28.6732% 17867 104154 17.1544%
exonic;splicing 33 188 17.5532% 0 0 0%
exonic_unknown 30 582 5.1546% 0 82 0%
frameshift_deletion 0 0 0% 1156 15977 7.2354%
frameshift_insertion 0 0 0% 993 13308 7.4617%
intergenic 4217805 15129055 27.8788% 364283 2238383 16.2744%
intronic 5249502 17735594 29.5987% 474650 2641780 17.9671%
ncRNA_exonic 111171 400185 27.7799% 8842 54342 16.271%
ncRNA_exonic;splicing 85 231 36.7965% 8 43 18.6047%
ncRNA_intronic 1058506 3728327 28.3909% 96152 575920 16.6954%
ncRNA_splicing 670 2341 28.6202% 86 478 17.9916%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 687 8777 7.8273%
nonframeshift_insertion 0 0 0% 299 4784 6.25%
nonsynonymous 52299 336233 15.5544% 0 0 0%
splice_acceptor 118 750 15.7333% 111 964 11.5145%
splice_donor 138 1076 12.8253% 44 763 5.7667%
splice_donor_acceptor 0 0 0% 20 45 44.4444%
splice_UTR5 93 400 23.25% 9 106 8.4906%
splie_Others 0 0 0% 53 654 8.104%
startloss 164 671 24.4411% 8 51 15.6863%
stopgain 585 4175 14.012% 34 1252 2.7157%
stoploss 87 353 24.6459% 7 63 11.1111%
synonymous 114048 548813 20.7808% 0 0 0%
upstream 192086 679592 28.2649% 16306 98760 16.5107%
upstream;downstream 16420 57451 28.5809% 1517 9367 16.1952%
UTR3 94541 361040 26.1857% 9685 61415 15.7698%
UTR5 27788 112990 24.5933% 2257 16935 13.3274%
UTR5;UTR3 681 2524 26.981% 66 381 17.3228%
Total 11323356 39753026 28.4843% 995141 5848802 17.0144%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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