Gold Sicilian Buttercup
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Gold Sicilian Buttercup
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region Italy
Purpose Fancy
Description

The Sicilian Buttercup is a rare breed originating from the island of Sicily, Italy. The name is from the unusual cup-shaped comb. The breed was imported to the United States in the 19th Century, and to Britain and Australia early in the 20th century. As of 1918, the Gold Sicilian Buttercups were admitted into the Standard of Perfection with the American Poultry Association.

Characteristics

Buttercup roosters and hens have completely different plumage patterns. The roosters sport rich deep orange feathers with a greenish black tail. While the hens are of a gold color, with black spangles running in parallel rows producing an almost spotty appearance. The earlobes tend to be white and the legs are of a soft green color. The Buttercup hen lays well (around 180 white eggs per year), but she isn't known for going broody. On avarage, the weights for rooster and hen are 3 kg and 2.5 kg, respectively.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 60065 650455 9.2343% 5886 104154 5.6512%
exonic;splicing 11 188 5.8511% 0 0 0%
exonic_unknown 2 582 0.3436% 0 82 0%
frameshift_deletion 0 0 0% 374 15977 2.3409%
frameshift_insertion 0 0 0% 380 13308 2.8554%
intergenic 1355800 15129055 8.9616% 120337 2238383 5.3761%
intronic 1703947 17735594 9.6075% 155434 2641780 5.8837%
ncRNA_exonic 36485 400185 9.117% 2932 54342 5.3955%
ncRNA_exonic;splicing 28 231 12.1212% 2 43 4.6512%
ncRNA_intronic 354135 3728327 9.4985% 32745 575920 5.6857%
ncRNA_splicing 230 2341 9.8249% 29 478 6.0669%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 182 8777 2.0736%
nonframeshift_insertion 0 0 0% 99 4784 2.0694%
nonsynonymous 15838 336233 4.7104% 0 0 0%
splice_acceptor 26 750 3.4667% 29 964 3.0083%
splice_donor 43 1076 3.9963% 13 763 1.7038%
splice_donor_acceptor 0 0 0% 11 45 24.4444%
splice_UTR5 24 400 6% 3 106 2.8302%
splie_Others 0 0 0% 11 654 1.682%
startloss 63 671 9.389% 2 51 3.9216%
stopgain 172 4175 4.1198% 7 1252 0.5591%
stoploss 29 353 8.2153% 1 63 1.5873%
synonymous 37092 548813 6.7586% 0 0 0%
upstream 61836 679592 9.099% 5188 98760 5.2531%
upstream;downstream 5156 57451 8.9746% 438 9367 4.676%
UTR3 29496 361040 8.1697% 3268 61415 5.3212%
UTR5 8784 112990 7.7741% 730 16935 4.3106%
UTR5;UTR3 217 2524 8.5975% 24 381 6.2992%
Total 3669479 39753026 9.2307% 328126 5848802 5.6101%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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