Dulong chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Dulong chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

Dulong chicken is named because it is raised by the Dulong people. It is a local breed used for meat and eggs.The origin and distribution area of Dulong chickens are Dulongjiang Township, Gongshan County, Yunnan Province, China. In the 1990s, there were about 20,000 individuals, but in recent years there has been a decline. In 2008, there were about 14,000 chickens.

Characteristics

Dulong chicken bodies are small. The feather color is white, yellow, black, or dark slate grey. The beaks are black or black with yellow, a few are light yellow. They have red single comb, a few are bean comb or rose comb. The earlobes are red. The skin are white. The color of shank is black or dark slate grey, followed by pink, yellow. Most individuals have shank feathers. They are not good layers, they start laying at 210-240 days and lay 55-75 eggs a year.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 127378 650455 19.5829% 10937 104154 10.5008%
exonic;splicing 19 188 10.1064% 0 0 0%
exonic_unknown 8 582 1.3746% 0 82 0%
frameshift_deletion 0 0 0% 334 15977 2.0905%
frameshift_insertion 0 0 0% 288 13308 2.1641%
intergenic 3209678 15129055 21.2153% 250785 2238383 11.2038%
intronic 3991412 17735594 22.5051% 333587 2641780 12.6274%
ncRNA_exonic 81403 400185 20.3413% 5988 54342 11.0191%
ncRNA_exonic;splicing 63 231 27.2727% 4 43 9.3023%
ncRNA_intronic 819327 3728327 21.9757% 68375 575920 11.8723%
ncRNA_splicing 490 2341 20.9312% 44 478 9.205%
ncRNA_UTR5 0 0 0% 0 18 0%
nonframeshift_deletion 0 0 0% 262 8777 2.9851%
nonframeshift_insertion 0 0 0% 88 4784 1.8395%
nonsynonymous 27413 336233 8.153% 0 0 0%
splice_acceptor 74 750 9.8667% 51 964 5.2905%
splice_donor 82 1076 7.6208% 19 763 2.4902%
splice_donor_acceptor 0 0 0% 10 45 22.2222%
splice_UTR5 38 400 9.5% 4 106 3.7736%
splie_Others 0 0 0% 25 654 3.8226%
startloss 73 671 10.8793% 1 51 1.9608%
stopgain 235 4175 5.6287% 13 1252 1.0383%
stoploss 40 353 11.3314% 3 63 4.7619%
synonymous 67914 548813 12.3747% 0 0 0%
upstream 103843 679592 15.2802% 7654 98760 7.7501%
upstream;downstream 6576 57451 11.4463% 552 9367 5.893%
UTR3 65301 361040 18.0869% 6182 61415 10.0659%
UTR5 11438 112990 10.123% 692 16935 4.0862%
UTR5;UTR3 316 2524 12.5198% 31 381 8.1365%
Total 8513121 39753026 21.415% 685929 5848802 11.7277%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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