Dehua Black chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Dehua Black chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Broiler
Description

Dehua black chickens are a meat type local breed. Dehua black chickens originated in Dehua County, Quanzhou City, Fujian Province, China. It has been developed more than 260 years.

Characteristics

Dehua black chickens are medium in size and have a upright single comb. They have black feathers with dark green metallic luster all over their body. They have no shank feathers and have four toes. According to the color of the comb, they can be divided into two breeds, the Black-comb breed have purple black combs, face, earlobes and wattles. The skin, meat, bones and internal organs are black. The Red-comb breed have purple combs, face, earlobes and wattles. The skin, meat and bones are white.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 168903 650455 25.9669% 15409 104154 14.7944%
exonic;splicing 33 188 17.5532% 0 0 0%
exonic_unknown 34 582 5.8419% 0 82 0%
frameshift_deletion 0 0 0% 871 15977 5.4516%
frameshift_insertion 0 0 0% 743 13308 5.5831%
intergenic 3862395 15129055 25.5297% 319367 2238383 14.2678%
intronic 4813551 17735594 27.1406% 419547 2641780 15.8812%
ncRNA_exonic 101086 400185 25.2598% 7677 54342 14.1272%
ncRNA_exonic;splicing 73 231 31.6017% 3 43 6.9767%
ncRNA_intronic 968923 3728327 25.9881% 84930 575920 14.7468%
ncRNA_splicing 614 2341 26.2281% 84 478 17.5732%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 516 8777 5.879%
nonframeshift_insertion 0 0 0% 232 4784 4.8495%
nonsynonymous 44707 336233 13.2964% 0 0 0%
splice_acceptor 96 750 12.8% 91 964 9.4398%
splice_donor 120 1076 11.1524% 31 763 4.0629%
splice_donor_acceptor 0 0 0% 16 45 35.5556%
splice_UTR5 77 400 19.25% 8 106 7.5472%
splie_Others 0 0 0% 39 654 5.9633%
startloss 147 671 21.9076% 4 51 7.8431%
stopgain 464 4175 11.1138% 20 1252 1.5974%
stoploss 79 353 22.3796% 7 63 11.1111%
synonymous 101510 548813 18.4963% 0 0 0%
upstream 173436 679592 25.5206% 13974 98760 14.1495%
upstream;downstream 14429 57451 25.1153% 1239 9367 13.2273%
UTR3 84178 361040 23.3154% 8301 61415 13.5162%
UTR5 24485 112990 21.6701% 1866 16935 11.0186%
UTR5;UTR3 591 2524 23.4152% 51 381 13.3858%
Total 10359931 39753026 26.0607% 875027 5848802 14.9608%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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