Croad Langshan
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Croad Langshan
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region Europe
Purpose Layer
Description

Croad Langshans are one of the most ancient chicken breeds. They originate from Rudong, eastern China. The first birds to reach the western world were purchased in 1871 at the Langshan Crossing near Shanghai, and sent to Major F.T. Croad by a relative residing in China. From these birds and some subsequent imports his niece, Miss Croad established the breed in England.

Characteristics

Croad Langshans are very large. Rooster can stand 90cm tall. The proud stance with tail carried high is very characteristic for roosters and hens. They have thick, glossy very soft plumage, and their legs are feathered. The most common colour is black with a beetle green sheen but there are also white Croads. Young Croad Langshan hens lay about 150 eggs a year and get broody. Egg colour is variable, but mostly buff with a purple bloom.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 117104 650455 18.0034% 10719 104154 10.2915%
exonic;splicing 24 188 12.766% 0 0 0%
exonic_unknown 23 582 3.9519% 0 82 0%
frameshift_deletion 0 0 0% 652 15977 4.0809%
frameshift_insertion 0 0 0% 606 13308 4.5537%
intergenic 2631912 15129055 17.3964% 218191 2238383 9.7477%
intronic 3341272 17735594 18.8394% 290093 2641780 10.981%
ncRNA_exonic 70098 400185 17.5164% 5386 54342 9.9113%
ncRNA_exonic;splicing 48 231 20.7792% 4 43 9.3023%
ncRNA_intronic 672192 3728327 18.0293% 59259 575920 10.2894%
ncRNA_splicing 411 2341 17.5566% 46 478 9.6234%
ncRNA_UTR5 0 0 0% 0 18 0%
nonframeshift_deletion 0 0 0% 359 8777 4.0902%
nonframeshift_insertion 0 0 0% 167 4784 3.4908%
nonsynonymous 30392 336233 9.039% 0 0 0%
splice_acceptor 58 750 7.7333% 74 964 7.6763%
splice_donor 71 1076 6.5985% 33 763 4.325%
splice_donor_acceptor 0 0 0% 14 45 31.1111%
splice_UTR5 60 400 15% 6 106 5.6604%
splie_Others 0 0 0% 27 654 4.1284%
startloss 104 671 15.4993% 3 51 5.8824%
stopgain 331 4175 7.9281% 20 1252 1.5974%
stoploss 51 353 14.4476% 3 63 4.7619%
synonymous 71853 548813 13.0924% 0 0 0%
upstream 119210 679592 17.5414% 9460 98760 9.5788%
upstream;downstream 10050 57451 17.4932% 868 9367 9.2666%
UTR3 58826 361040 16.2935% 6037 61415 9.8298%
UTR5 16135 112990 14.28% 1196 16935 7.0623%
UTR5;UTR3 389 2524 15.412% 39 381 10.2362%
Total 7140614 39753026 17.9624% 603262 5848802 10.3143%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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