Combatiente
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Combatiente
Species taxonomy Gallus gallus domesticus
Classification Commercial
Region Spain
Purpose Fighting
Description

The Combatiente is a game fowl breed which was developed in the 18th century in Andalusia, Spain. It was from birds originally brought from Southeast Asia. The Combatiente rooster has traditionally been used for cockfighting.

Characteristics

There are three types of Combatiente Espanol chickens: the Spanish Fighter; the Jerezano Spanish Fighter, and the Canario Spanish Fighter. The three types were developed in different locations.There are more than four varieties of the plumage: Colorados (Partridge), Pinto (Black and White), Melado (Chestnut), Giro (Gold Duckwing).The Combatiente's weight is usualy 460-1800 g. Roosters are highly aggressive and must be separated.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 151172 650455 23.241% 13382 104154 12.8483%
exonic;splicing 35 188 18.617% 0 0 0%
exonic_unknown 28 582 4.811% 0 82 0%
frameshift_deletion 0 0 0% 939 15977 5.8772%
frameshift_insertion 0 0 0% 841 13308 6.3195%
intergenic 3457677 15129055 22.8545% 281078 2238383 12.5572%
intronic 4308730 17735594 24.2943% 366091 2641780 13.8577%
ncRNA_exonic 90822 400185 22.695% 6719 54342 12.3643%
ncRNA_exonic;splicing 69 231 29.8701% 7 43 16.2791%
ncRNA_intronic 863353 3728327 23.1566% 74496 575920 12.9351%
ncRNA_splicing 531 2341 22.6826% 70 478 14.6444%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 486 8777 5.5372%
nonframeshift_insertion 0 0 0% 241 4784 5.0376%
nonsynonymous 42235 336233 12.5612% 0 0 0%
splice_acceptor 77 750 10.2667% 72 964 7.4689%
splice_donor 117 1076 10.8736% 28 763 3.6697%
splice_donor_acceptor 0 0 0% 12 45 26.6667%
splice_UTR5 72 400 18% 6 106 5.6604%
splie_Others 0 0 0% 41 654 6.2691%
startloss 134 671 19.9702% 3 51 5.8824%
stopgain 432 4175 10.3473% 27 1252 2.1565%
stoploss 66 353 18.6969% 6 63 9.5238%
synonymous 104152 548813 18.9777% 0 0 0%
upstream 155517 679592 22.8839% 12083 98760 12.2347%
upstream;downstream 13099 57451 22.8003% 1079 9367 11.5192%
UTR3 75374 361040 20.8769% 7362 61415 11.9873%
UTR5 21361 112990 18.9052% 1552 16935 9.1645%
UTR5;UTR3 517 2524 20.4834% 51 381 13.3858%
Total 9285570 39753026 23.3581% 766673 5848802 13.1082%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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