Nixi chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Nixi chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

The Nixi Chicken is a local breed used for meat and eggs. The origin and mainly distribution areas of Nixi Chicken are Benzilan and Yangla in Deqin County, Deqing Prefecture, Yunnan Province, Nixi and Dongwang in Shangri-la County, Yunnan Province, and Guxue and Jiefang in Derong County, Ganzi Prefecture, Sichuan Province, China.

Characteristics

The Nixi chickens are small in body size, with its head and tail upturned. They have erect single comb, the combs, wattles, and earlobes are red, with a few in purple. The beaks are black, and a few are yellowish, bluish or iron-gray. The shanks are black, green, or yellow. The skins are mostly white and a few black. They start laying at 180-210 days and lay 170-180 eggs a year.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 113147 650455 17.3951% 9395 104154 9.0203%
exonic;splicing 15 188 7.9787% 0 0 0%
exonic_unknown 10 582 1.7182% 0 82 0%
frameshift_deletion 0 0 0% 254 15977 1.5898%
frameshift_insertion 0 0 0% 242 13308 1.8185%
intergenic 2868738 15129055 18.9618% 219496 2238383 9.806%
intronic 3591467 17735594 20.2501% 293717 2641780 11.1181%
ncRNA_exonic 73539 400185 18.3763% 5341 54342 9.8285%
ncRNA_exonic;splicing 60 231 25.974% 4 43 9.3023%
ncRNA_intronic 749181 3728327 20.0943% 61071 575920 10.6041%
ncRNA_splicing 451 2341 19.2653% 56 478 11.7155%
ncRNA_UTR5 0 0 0% 0 18 0%
nonframeshift_deletion 0 0 0% 208 8777 2.3698%
nonframeshift_insertion 0 0 0% 85 4784 1.7768%
nonsynonymous 23311 336233 6.933% 0 0 0%
splice_acceptor 65 750 8.6667% 50 964 5.1867%
splice_donor 75 1076 6.9703% 18 763 2.3591%
splice_donor_acceptor 0 0 0% 11 45 24.4444%
splice_UTR5 31 400 7.75% 3 106 2.8302%
splie_Others 0 0 0% 23 654 3.5168%
startloss 67 671 9.9851% 0 51 0%
stopgain 208 4175 4.982% 9 1252 0.7188%
stoploss 42 353 11.898% 3 63 4.7619%
synonymous 59256 548813 10.7971% 0 0 0%
upstream 92410 679592 13.5979% 6550 98760 6.6322%
upstream;downstream 5626 57451 9.7927% 451 9367 4.8148%
UTR3 56896 361040 15.7589% 5268 61415 8.5777%
UTR5 10140 112990 8.9742% 619 16935 3.6552%
UTR5;UTR3 245 2524 9.7068% 25 381 6.5617%
Total 7644980 39753026 19.2312% 602899 5848802 10.3081%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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