Shouguang chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Shouguang chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

Shouguang chicken, also known as "Cilun chicken", is a local breed used for meat and eggs. Shouguang chickens origin for Daotian Town, Shouguang City, Shandong Province, China.

Characteristics

Shouguang chickens are tall and strong. The whole body feathers are black, with metal luster. The tail can be divided into long and short. The comb types are single comb. The combs, wattles, earlobes, faces are red, the beaks, shanks, toes are black, the skins are white. On avarage, the weights for rooster and hen are 3.25 kg and 2.8 kg, respectively.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 93626 650455 14.3939% 8093 104154 7.7702%
exonic;splicing 13 188 6.9149% 0 0 0%
exonic_unknown 29 582 4.9828% 0 82 0%
frameshift_deletion 0 0 0% 237 15977 1.4834%
frameshift_insertion 0 0 0% 199 13308 1.4953%
intergenic 2377529 15129055 15.715% 184592 2238383 8.2467%
intronic 2982813 17735594 16.8182% 246618 2641780 9.3353%
ncRNA_exonic 61158 400185 15.2824% 4515 54342 8.3085%
ncRNA_exonic;splicing 37 231 16.0173% 4 43 9.3023%
ncRNA_intronic 624049 3728327 16.738% 51739 575920 8.9837%
ncRNA_splicing 355 2341 15.1645% 38 478 7.9498%
ncRNA_UTR5 0 0 0% 1 18 5.5556%
nonframeshift_deletion 0 0 0% 190 8777 2.1647%
nonframeshift_insertion 0 0 0% 66 4784 1.3796%
nonsynonymous 19351 336233 5.7552% 0 0 0%
splice_acceptor 47 750 6.2667% 35 964 3.6307%
splice_donor 57 1076 5.2974% 16 763 2.097%
splice_donor_acceptor 0 0 0% 12 45 26.6667%
splice_UTR5 30 400 7.5% 2 106 1.8868%
splie_Others 0 0 0% 18 654 2.7523%
startloss 44 671 6.5574% 1 51 1.9608%
stopgain 179 4175 4.2874% 14 1252 1.1182%
stoploss 32 353 9.0652% 3 63 4.7619%
synonymous 49037 548813 8.9351% 0 0 0%
upstream 74620 679592 10.9801% 5450 98760 5.5184%
upstream;downstream 4575 57451 7.9633% 357 9367 3.8113%
UTR3 47645 361040 13.1966% 4386 61415 7.1416%
UTR5 8211 112990 7.267% 499 16935 2.9466%
UTR5;UTR3 195 2524 7.7258% 10 381 2.6247%
Total 6343632 39753026 15.9576% 507095 5848802 8.6701%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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