Aijiao chicken
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Background Information Variants Annotation&Density Genetic Differentiation
Genetic Affinity ADMIXTURE Analysis Runs of Homozygosity
Linkage Disequilibrium Decay Demographic History Selection

Background Information

Breed Card
Breed Aijiao chicken
Species taxonomy Gallus gallus domesticus
Classification Traditional
Region China
Purpose Dual
Description

The Aijiao chicken is a local breed used for producing eggs and meat. The Aijiao chicken originated from Xingyi City, Guizhou Province, China. It has been kept for more than 200 years and has now become an endangered breed even in the source area.

Characteristics

Aijiao chickens are small in size, and the shanks are short. It is a bantam breed. The plumage colour is yellow or light black. Their beaks are short and the colour is yellow or grayish black. They have red single combs. The ears and wattles are red. The skins are white. The shanks are yellow, white or black. The hens start laying at 180-210 days and lay 160-190 eggs aa year.


Samples Information

Variants Annotation&Density

Annotation Population SNP Total SNP Percentage SNP Population INDEL Total INDEL Percentage INDEL
downstream 102412 650455 15.7447% 8503 104154 8.1639%
exonic;splicing 18 188 9.5745% 0 0 0%
exonic_unknown 20 582 3.4364% 0 82 0%
frameshift_deletion 0 0 0% 375 15977 2.3471%
frameshift_insertion 0 0 0% 327 13308 2.4572%
intergenic 2016557 15129055 13.329% 147862 2238383 6.6058%
intronic 2752216 17735594 15.518% 216199 2641780 8.1838%
ncRNA_exonic 54515 400185 13.6224% 3704 54342 6.8161%
ncRNA_exonic;splicing 44 231 19.0476% 3 43 6.9767%
ncRNA_intronic 474109 3728327 12.7164% 36717 575920 6.3754%
ncRNA_splicing 312 2341 13.3276% 40 478 8.3682%
ncRNA_UTR5 0 0 0% 0 18 0%
nonframeshift_deletion 0 0 0% 248 8777 2.8256%
nonframeshift_insertion 0 0 0% 102 4784 2.1321%
nonsynonymous 25671 336233 7.6349% 0 0 0%
splice_acceptor 60 750 8% 38 964 3.9419%
splice_donor 82 1076 7.6208% 14 763 1.8349%
splice_donor_acceptor 0 0 0% 6 45 13.3333%
splice_UTR5 46 400 11.5% 4 106 3.7736%
splie_Others 0 0 0% 24 654 3.6697%
startloss 86 671 12.8167% 2 51 3.9216%
stopgain 268 4175 6.4192% 12 1252 0.9585%
stoploss 44 353 12.4646% 5 63 7.9365%
synonymous 62464 548813 11.3817% 0 0 0%
upstream 99939 679592 14.7057% 7194 98760 7.2843%
upstream;downstream 8762 57451 15.2513% 682 9367 7.2809%
UTR3 54965 361040 15.2241% 4922 61415 8.0143%
UTR5 13094 112990 11.5886% 761 16935 4.4937%
UTR5;UTR3 346 2524 13.7084% 43 381 11.2861%
Total 5666030 39753026 14.2531% 427787 5848802 7.3141%
SNP Density
SNP_Annotation
INDEL Density
INDEL_Annotation

Genetic Differentiation

Summary

Genetic affinities of target population in the context of worldwide populations are measured by pairwise FST between target population and references. Smaller FST value indicates closer relationship. Regions represented by different colors are indicated above.

Genetic Affinity

PCA for G.gallus without G.g.bankiva , G.g.jabouillei, and some G.g.gallus

Summary

Genetic affiliation and population structure are shown by PCA plots. After removing G. g. bankiva, G. g. jabouillei, and some G. g. gallus individuals as outliers to other Red Jungle Fowl, the dataset contains 1,915 samples from domestic chicken and Red Jungle Fowl. User can add any populations with interests to show with the target population under this PCA context by using the item of “Add”.

ADMIXTURE Analysis

Summary

The inference of populations and individual ancestries is revealed by ADMIXTURE clustering. Length of each colored bar represents the proportion of proposed ancestry in the sample. User can add any populations with interests to compare with the target population by using the item of “Add”. The number of proposed ancestries is determined by “which K”.

Runs of Homozygosity

Summary

Runs of homozygosity (ROH) indicates long tracts of homozygous genotypes inherited from identical haplotypes of a common ancestor. Larger populations have fewer, shorter ROH, whereas isolated or bottlenecked populations have more, somewhat longer ROH. Admixture brings the fewest ROH, whereas inbreeding causes long ROH. The level of ROH is measured by number and length. The length of ROH can be defined in “ROH range”. User can add any populations with interests to compare with the target population by using the item of “Add”.

Linkage Disequilibrium Decay

Summary

Linkage disequilibrium (LD) decay is characterized by squared correlations (r²) of all SNPs frequencies against the physical distances between SNPs. User can add any populations with interests to compare with the target population by using the item of “Add”.

Demographic History

Summary

The changes of effective population size through time is inferred by SMC++. User can add any populations with interests to compare with the target population by using the item of “Add”.

Selection

Summary

Selective signals of the target population are detected by different methods. X axis indicates physical position of specific chromosomal region with interests which can be defined by “Region”. The gene annotation is shown below. Y axis of left (YL) indicates the values of Pi-ratio of -log2(πRJF/πTarget) or composite likelihood ratio (CLR) of SweeD. The levels of statistical significance are noted with different colors. Y axis of right (YR) shows the values of Fst (Target vs. Red Jungle Fowl), Pi, or Tajima’s D with blue line with sliding window approach. The genomic window size and step size for Tajima’s D are 5 kb and 5 kb, respectively. The genomic window size and step size for Fst and Pi are 10 kb and 5 kb, respectively. The methods are defined by “Method-YL” and “Method-YR”.

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